Crocs: Cross-Technology Clock Synchronization for WiFi and ZigBee

Clock synchronization is a key function in embedded wireless systems and networks. This issue is equally important and more challenging in IoT systems nowadays, which often include heterogeneous wireless devices that follow different wireless standards. Conventional solutions to this problem employ gateway-based indirect synchronization, which suffers low accuracy. This paper for the first time studies the problem of cross-technology clock synchronization. Our proposal called Crocs synchronizes WiFi and ZigBee devices by direct cross-technology communication. Crocs decouples the synchronization signal from the transmission of a timestamp. By incorporating a barker-code based beacon for time alignment and cross-technology transmission of timestamps, Crocs achieves robust and accurate synchronization among WiFi and ZigBee devices, with the synchronization error lower than 1 millisecond. We further make attempts to implement different cross-technology communication methods in Crocs and provide insight findings with regard to the achievable accuracy and expected overhead

Fluorescent Nanoparticles from Several Commercial Beverages: Their Properties and Potential Application for Bioimaging

The presence of nanoparticles in beverages has raised great concern in terms of potential impacts to consumer health. Herein, carbon dots in beverages kvass, pony malta, pilsner beer, Vivant Storm, and Profit were identified. They were shown to have a strong fluorescence under the excitation of ultraviolet light. The emission peaks shift to longer wavelengths accompanied by a remarkable fluorescence intensity decrease. The carbon dots are in the nanosized range and roughly spherical in appearance. Elemental analysis by X-ray photoelectron spectroscopy demonstrated the composition of Kvass carbon dots to be C 83.17%, O 13.83%, and N 3.00%. No cytotoxicity was found at concentrations up to 20 mg/mL for human tongue squamous carcinoma cells, and they can be directly applied in both carcinoma and onion epidermal cell imaging. This work represents the first report of the carbon dots present in beverages, providing valuable insights into these nanoparticles for future biological imaging

Development of o-aminobenzamide salt derivatives for improving water solubility and anti-undifferentiated gastric cancer

Background: Gastric cancer is one of the cancers with wide incidence, difficult treatment and high mortality in the world, especially in Asia and Africa. In our previous work, a novel o-aminobenzamide analogue F8 was identified as an early preclinical candidate for treatment of undifferentiated gastric cancer (IC50 of 0.26 μM for HGC-27). However, the poor water solubility of compound F8 prevents its further progress in preclinical studies.Aim: To improve the water solubility and drug-likeness of F8 via salt formation.Method: Different acids and F8 were reacted to obtain different salt forms. Physicochemical property screening, pharmacokinetic property research, and antitumor biological activity evaluation in vitro and in vivo were used to obtain the optimal salt form with the best druggability.Results: our continuous efforts have finally confirmed F8·2HCl as the optimal salt form with maintained in vitro antitumor activity, improved water solubility and pharmacokinetic properties. Importantly, the F8·2HCl displayed superior in vivo antitumor efficacy (TGI of 70.1% in 75 mg/kg) in HGC-27 xenograft model. The further immunohistochemical analysis revealed that F8·2HCl exerts an antitumor effect through the regulation of cell cycle-related protein (CDK2 and p21), apoptosis-related protein Cleaved Caspase-3, proliferation marker Ki67, and cell adhesion molecule E-cadherin. In addition, F8·2HCl showed acceptable safety in the in vivo acute toxicity assay.Conclusion: Salting is an effective means to improve the drug-like properties of compound F8, and F8·2HCl can serve as a promising therapeutic agent against undifferentiated gastric cancer

Anthocyanins from the Red Juvenile Leaves of Loropetalum Chinense var. rubrum (Chinese Fringe Flower) Identification and pH Sensing Behaviors

In this study, anthocyanins were extracted from the frozen juvenile leaves of Loropetalum Chinense var. rubrum with high efficiency using ethanol containing 0.1% HCl as extractant. After being purified with AB-8 resin, this extract was investigated with HPLC-DAD-MS/MS determination, and six anthocyanins were identified according to chromatogram, MS data and ratios of absorbance at 400-460 nm to that at absorption maximum (~520 nm), A400-460/A520. Besides cyanidin 3-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, the most abundant malvidin 3,5-O-diglucoside was confirmed through preparative HPLC separation and 1 H NMR determination. However, two delphinidin- and petunidin-derived anthocyanins should be further clarified whether they are 3,5-O-diglucoside or 5-O-glucobioside. In addition, a novel anthocyanin, 2H-pyran[5,6]malvidin 3-O-glucobioside was also proposed yet need more data to confirm. Moreover, malvidin 3,5-O-diglucoside showed excellent pH sensing ability, displaying distinct color change from pink (pH 1.5) to colorlessness (pH 5.5), to blue (pH 6.8) and finally green (pH 8.8 to pH 10.5). All these should be helpful to evaluate this plant as natural pigment resource. </p

Experimental Realization of a Skyrmion Circulator

Magnetic skyrmions are mobile topological spin textures that can be manipulated by different means. Their applications have been frequently discussed in the context of information carriers for racetrack memory devices, which on the other hand, exhibit a skyrmion Hall effect as a result of the nontrivial real-space topology. While the skyrmion Hall effect is believed to be detrimental for constructing racetrack devices, we show here that it can be implemented for realizing a three-terminal skyrmion circulator. In analogy to the microwave circulator, nonreciprocal transportation and circulation of skyrmions are studied both numerically and experimentally. In particular, successful control of the circulating direction of being either clockwise or counterclockwise is demonstrated, simply by changing the sign of the topological charge. Our studies suggest that the topological property of skyrmions can be incorporated for enabling novel spintronic functionalities; the skyrmion circulator is just one example

Association between Religion and Health in China: Using Propensity Score Matching Method

The association between religion and health is well debated and receives continuous attention in research. Selection bias is often a major concern among the observatory data routinely used worldwide to examine this topic. Adopting the propensity score matching (PSM) method, the present study tries to assess the treatment effects of religion on self-reported health status. The final sample from the 2007 Spiritual Life Study of Chinese Residents (SLSC) contains 6194 valid responses. The average treatment effects (ATEs) estimated by the PSM method show that respondents with religious affiliations are on average significantly more likely to report being very healthy by 5.2 percentage points (by 3.6 and 9.6 percentage points among Buddhists and Protestants), especially, by 16.2 percentage points among those regarding religion as being very important in their lives. Meanwhile, ATEs of religion on reporting being very happy is 17.0 among Protestants and 13.4 among those regarding religion with high importance and 11.3 among those with &ldquo;regular religious attendance&rdquo;

Calpain activation and disturbance of autophagy are induced in cortical neurons in vitro by exposure to HA/β-Ga2O3:Cr3+ nanoparticles

The toxicity of engineered nanoparticles remains a concern. The knowledge of biohazards associated with particular nanoparticles is crucial to make this cutting-edge technology more beneficial and safe. Here, we evaluated the toxicity of Ga2O3 nanoparticles (NPs), which are frequently used to enhance the performance of metal catalysts in a variety of catalytic reactions. The potential inflammatory signaling associated with the toxicity of HA/β-Ga2O3:Cr3+ NPs in primary cortical neurons was examined. We observed a dose-dependent decrease in cell viability and an increase in apoptosis in neurons following various concentrations (0, 1, 5, 25, 50, 100 µg/ml) of HA/β-Ga2O3:Cr3+ NPs treatment. Consistently, constitutively active forms of calcineurin (48 kDa) were significantly elevated in cultured primary cortical neurons, which was consistent with calpain activation indicated by the breakdown products of spectrin. Moreover, HA/β-Ga2O3:Cr3+ NPs result in the elevation of LC3-II formation, SQSTM/p62, and Cathepsin B, whereas phosphorylation of CaMKII (Thr286) and Synapsin I (Ser603) were downregulated in the same context. Taken together, these results demonstrate for the first time that calpain activation and a disturbance of autophagy signaling are evoked by exposure to HA/β-Ga2O3:Cr3+ NPs, which may contribute to neuronal injury in vitro

Fluorescent nanoparticles present in Coca-Cola and Pepsi-Cola: physiochemical properties, cytotoxicity, biodistribution and digestion studies

<p>Foodborne nanoparticles (NPs) have drawn great attention due to human health concerns. This study reports the detection of the presence of fluorescent NPs, about 5 nm, in two of the most popular beverages, Coca-Cola (Coke) and Pepsi-Cola (Pepsi). The NPs contain H, C and O, three elements with a tunable emission and with a quantum yield of 3.3 and 4.3% for Coke and Pepsi, respectively. The presence of sp³-hybridized carbon atoms of alcohols and ethers bonds was confirmed by NMR analysis. The NPs can be taken up by living cells and accumulate within cell membrane and cytoplasm. Evaluation of the acute toxicity of the NPs revealed that the BALB/c mice appeared healthy after administration of a single dose of 2 g kg⁻¹ body weight. Analysis of glutamate pyruvate transaminase (GPT), glutamic oxaloacetic transaminase (GOT), urea and creatinine showed that there were statistically, but not biologically, significant differences in some of these biochemical parameters between the test and control groups. No obvious organ damage or apparent histopathological abnormality was observed in the tested mice. The biodistribution study in major organs indicated that the NPs were easily accumulated in the digestive tract, and they were able to cross the blood–brain barrier and dispersed in the brain. <i>In vitro</i> digestion of the NPs showed a significant fluorescence quenching of the NPs. This work represents the first report of foodborne fluorescent NPs present in Coke and Pepsi, and provides valuable insights into physicochemical properties of these NPs and their toxicity characteristics both <i>in vitro</i> and <i>in vivo</i>.</p